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Flow cytometer

Scientific background

Monitoring phytoplankton in the Belgian part of the North Sea on a near-continuous basis can yield valuable information on the ecological status of these waters. The use of a flow cytometer can improve this understanding and increase the efficiency of analyses and reporting. Flow cytometry is a commonly used technique among biologists to study temporal and spatial changes of phytoplankton species composition and abundance. It creates fingerprints of particles (phytoplankton cells) based on their ability to scatter or re-emit specific wavelengths of light. This light is detected by sensors and by analyzing fluctuations in brightness it is then possible to derive information about the physical and chemical structure of each individual particle.

Images and cytograms of cultured phytoplankton species.

Infrastructure

In the framework of LifeWatch, VLIZ put a CytoSub flow cytometer (Cytobuoy) into service. It is installed on board in the laboratory space of the RV Simon Stevin and is connected to its continuous water flow system. The instrument is designed to analyze the naturally occurring size range from small (e.g. picoplankton) to large (e.g. colonial) plankton species (1 to 800 µm). In addition to its ability to gather flow cytometric data, the instrument can also take pictures of individual particles. This can allow for easier identification of particle clusters. The embedded computer of the CytoSub is connected to the network of the RV Simon Stevin, which allows off site operating the instrument via a remote desktop connection.

   

Left: flow cytometer being tested in the lab (©VLIZ) - Right: flow cytometer installed aboard the RV Simon Stevin (©VLIZ)

Data treatment

High-throughput flow cytometric data generated during the RV Simon Stevin campaigns are automatically processed with Easyclus LIVE©. Results are transferred via the ship’s satellite connection and visualized online.

 

Left: total red fluorescence plot - Right: scatter plot of clustered particles.

Applications

In addition to using the flow cytometer in function of general sampling campaigns, the instrument can be employed for a wide range of applications in the field of marine science. E.g. the screening of phytoplankton cultures, detection of harmful algal blooms (HABs), detection of microplastics and others.

 

Publications