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Carotenoid producing bacteria from Antarctica
Citation
Vila E, Hornero-Mendez D, Azziz G, Lareo C, Saravia V (2021): Carotenoid producing bacteria from Antarctica. v1.0. SCAR - Microbial Antarctic Resource System. Dataset/Occurrence. https://ipt.biodiversity.aq/resource?r=carotenoid_bacteria_antarctica&v=1.0 https://doi.org/10.15468/cur76e

Access data
Archived data
Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Notes: The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Description
A dataset of 30 bacterial cultures from coastal and marine areas in 2014 from sites in Fildes Peninsula, King George Island (Antarctica). Identification based on 16S ssu rRNA sequencing. more

A total of 32 liquid and solid samples were collected in 50 mL sterile tubes.
Environmental samples collected from Fildes Peninsula, King George Island, during the expedition organized by IAU (Uruguayan Antarctic Institute) on December 2014.
Approximately 100 mg of each sample was suspended in 900 μl of sterile NaCl 0.9% (w/v) solution, serially diluted and plated on adequate media. The isolation medium for samples of organic matter, sediments and ice water was Tryptic Soy Agar (TSA, Sigma Aldrich), and for sea water was TSA complemented with 20 g/L of sea salts (Sigma). Plates were incubated at 10 °C for 7–10 days, and colored colonies were selected for strain isolation by streak-plating technique. Once purity was verified, strains were conserved at −80 °C on glass beads with 20% glycerol in Tryptic Soy Broth (TSB, Oxoid) and sea salts when needed. Strains were characterized by colony and cell morphology, Gram staining and pigment composition.
Genomic DNA extraction was performed with a commercial kit according manufacturer’s instructions (Genomic DNA Purification Kit, Thermo Fisher). Amplification of the 16S rRNA gene fragments were done in a Palm-1870 Cycler TM (Corbett Research UK Ltd) as follows: initial denaturation 3 min at 95 °C, then 35 cycles of 45 s at 94 °C, 45 s at 58 °C, 60 s 72 °C, and a final extension step 9 min at 72 °C. Reaction mixtures contained: 2.5 U polymerase (Mango Taq, Bioline), 10 μL of buffer solution, 2.5 μL of 50 mM MgCl2, and 2.5 μL each of forward primer 27 F (5´-AGAGTTTGATC MTGGCTCAG-3´) and reverse primer 1492R (5´-TACGGYTACC TTGTTACGACTT-3´), genomic DNA, and water to 50 μL final volume. The PCR products were analyzed by electrophoresis with 1% agarose gels. DNA sequencing was carried out by Macrogen Inc. (Korea) using universal primers.

Scope
Themes:
Biology > Ecology - biodiversity
Keywords:
Marine/Coastal, Coastal, Cultures, Rrna, Sequencing, PSW, Antarctica, South Shetland I., King George I., Bacteria

Geographical coverage
PSW, Antarctica, South Shetland I., King George I. Stations [Marine Regions]
Fildes Peninsula

Temporal coverage
From December 2014 on [Completed]

Taxonomic coverage
Bacteria [WoRMS]

Parameter
Occurrence of biota

Contributors
University of the Republic of Uruguay; Faculty of Engineering; Instituto de Ingeniería Química, moredata creator

Related datasets
Published in:
AntOBIS: Antarctic Ocean Biodiversity Information System, more
(Partly) included in:
RAS: Register of Antarctic Species, more

Dataset status: Completed
Data type: Data
Data origin: Research: field survey
Metadatarecord created: 2021-07-05
Information last updated: 2021-07-05
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