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Assessment of imaging-in-flow system (FlowCAM) for systematic ballast water management
Romero-Martínez, L.; van Slooten, C.; Nebot, E.; Acevedo-Merino, A.; Peperzak, L. (2017). Assessment of imaging-in-flow system (FlowCAM) for systematic ballast water management. Sci. Total Environ. 603-604: 550-561.
In: Science of the Total Environment. Elsevier: Amsterdam. ISSN 0048-9697; e-ISSN 1879-1026, more
Peer reviewed article  

Available in  Authors 

Author keywords
    Phytoplankton; Exotic species; FlowCAM; Ballast water; Prorocentrum minimum; Flow cytometry

Authors  Top 
  • Romero-Martínez, L.
  • van Slooten, C., more
  • Nebot, E.
  • Acevedo-Merino, A.
  • Peperzak, L., more

    Assessing the disinfection of ballast water and its compliance with international standards requires determiningthe size, viability, and concentration of planktonic organisms. The FlowCAM(FlowCytometer andMicroscope) isan Imaging FlowCytometry designed to obtain the particle concentration, images, and quantitativemorphologicinformation. The objective in this paper is to establish the basis for transforming the FlowCAM from being a laboratoryanalyzer into a tool for systematic monitoring of ballast water. The capacity of the FlowCAM was evaluatedby analyzing artificial microbeads, phytoplankton monocultures, and real seawater samples. Microbeadanalyses reported high accuracy and precision in size and concentration measurements. Monoculture analysesshowed the effect of disinfection treatments in cell appearance and growth. Lowconcentration and heterogeneityof particles in real seawater analyses require the comprehensive observation of images by experts. Additionally,some physical characteristics of the device must be improved. The optimization of device configuration enablesthe quick transferring of files and information between parties involved in ballast watermanagement. FlowCAMmay become a feasible technology for this after the device and protocols are adapted.

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