Effects of salinity and dietary n-3 highly unsaturated fatty acids on the survival, development, and morphogenesis of the larvae of laboratory-reared mud crab Scylla serrata (Decapoda, Portunidae)
Dan, S.; Hamasaki, K. (2011). Effects of salinity and dietary n-3 highly unsaturated fatty acids on the survival, development, and morphogenesis of the larvae of laboratory-reared mud crab Scylla serrata (Decapoda, Portunidae). Aquacult. Int. 19(2): 323-338. https://dx.doi.org/10.1007/s10499-010-9374-z
In: Aquaculture International. Springer: London. ISSN 0967-6120; e-ISSN 1573-143X, more
Also appears in:Le Vay, L.; Zeng, C.; Sorgeloos, P. (Ed.) (2011). International Symposium on aquaculture, biology and management of commercially important crabs. Aquaculture International, 19(2). Springer: Dordrecht. 215-394 pp., more
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| Keywords |
Biological development > Larval development
Biological phenomena > Metamorphosis > Moulting
Developmental stages > Larvae
Laboratory culture
Morphogenesis
Population functions > Mortality
Properties > Chemical properties > Salinity
Symptoms
Decapoda [WoRMS]; Portunidae Rafinesque, 1815 [WoRMS]; Scylla serrata (Forskål, 1775) [WoRMS]
Marine/Coastal |
| Author keywords |
Larval morphogenesis; Moult death syndrome; Mud crab; n-3HUFA; Salinity;Seed production |
| Abstract |
We investigated the effects of feeding rotifers containing various levels of n-3 highly unsaturated fatty acids (n-3HUFA) to Scylla serrata larvae at different developmental stages on their survival, development, and morphogenesis when they were cultured at six salinity levels. The first-, third-, and fifth (last)-stage zoeae and megalopae were reared to first-stage crabs at salinities of 10, 15, 20, 25, 30, and 35aEuro degrees, with three different feeding regimes of rotifers containing different levels of n-3HUFA. The larvae successfully developed to the subsequent stages at 20-35aEuro degrees salinity. The highest survival rates to first-stage crabs were recorded at 20-25aEuro degrees salinity. The morphological features of the megalopa observed in the last-stage zoeae, represented by the ratio of the chela length to carapace length, tended to advance with increasing salinity, indicating higher assimilation efficiency at higher salinities. The megalopal features of the last-stage zoeae were enhanced when the larvae were fed rotifers containing higher amounts of docosahexaenoic acid (DHA). As reported previously, final-stage zoeal larvae with advanced megalopal features often experienced moult death syndrome (MDS). These results show that when larvae are fed rotifers with high DHA under high-salinity conditions, morphogenesis is accelerated, resulting in MDS. Therefore, to evaluate the effects of salinity on larval survival, it is necessary to examine larval morphogenesis in terms of MDS. In conclusion, we recommend that not only survival but also larval morphogenesis should be examined when evaluating the results of rearing experiments with S. serrata larvae. |
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