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Illuminating adaptive divergence through hybrid zones in Littorina saxatilis
Raffini, F.; Butlin, R.; Department of Animal and Plant Sciences. University of Sheffield: United Kingdom; (2019): Illuminating adaptive divergence through hybrid zones in Littorina saxatilis.

Availability: Unrestricted after moratorium period
Data are initially restricted, but the access condition relaxes to academic or unrestricted once a specified period of time after an event (such as collection, publication, completion of QC procedures or project cessation) has elapsed

Dataset describing position, habitat and date of collection, snail phenotype-genotype data, and positions and habitats at movement observation and recapture seks and developmental stage data. more

64 snails were collected in the field (Centinela, Baiona, Spain; coordinates: N 42° 4' 38.06", W 8° 53' 47.47") in the wave, crab and hybrid zone respectively (total sample size: 192). Prior to collection, the habitat and position of each individual was recorded with a survey instrument (total station) to <10cm resolution. Specimens were transported to the lab where they were marked with individual waterproof labels and non-toxic nail polish along the margin of the aperture (to track growth); shell thickness was measured with a gauge; shells photographed for morphology; and DNA collected from mucus (non-destructive DNA sampling protocol using TFA cards). Individuals were then released in the field: each collection group (crab/hybrid/wave) was randomly split in three subgroups and released in the hybrid, crab and wave habitats, respectively, recording the precise release position and habitat characteristics. One day later, the positions and habitats of marked snails was recorded for movement. This round of collection-mark-release was repeated four times (one release included only 64 snails from the hybrid zone due to not permissive weather), for a final number of 640 released snails. At the end of the visit (a total of 14 days, surviving snails plus three individuals released in a previous round of sampling in June were recaptured, with positions and habitats recorded, phenotyped (growth, weight) and dissected for sex determination and tissue collection for additional DNA analysis.

Biology > Benthos
Marine/Coastal, Adaptation, ASSEMBLEPlus Transnational Access, Data, divergent selection, genomic architecture, Habitat choice, Hybrid zones, Parallel evolution, Reproductive isolation, Speciation, Spain, Vigo, Littorina saxatilis (Olivi, 1792)

Geographical coverage
Spain, Vigo

Temporal coverage
13 June 2019 - 19 June 2019
21 September 2019 - 6 October 2019

Taxonomic coverage
Littorina saxatilis (Olivi, 1792) [WoRMS]

Body size, Developmental stages, Genotype, Growth, Habitat, Sex, Weight

University of Sheffield; School of Biological Sciences; Department of Animal and Plant Sciences (APS), moredata creator

Related datasets
Other relation:
Insights from hybrid zones: testing for habitat choice, divergent selection and genomic architecture in Littorina saxatilis adaptive divergence, more

ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, more

Dataset status: Completed
Data type: Data
Data origin: Research: field experiment
Metadatarecord created: 2019-12-03
Information last updated: 2020-04-16
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy