IMIS | Lifewatch regional portal

You are here

IMIS

[ report an error in this record ]basket (0): add | show Print this page

Isolation of planctomycetes from Aplysina sponges
Pimentel-Elardo, S.; Wehrl, M.; Friedrich, A.B.; Jensen, P.R.; Hentschel, U. (2003). Isolation of planctomycetes from Aplysina sponges. Aquat. Microb. Ecol. 33: 239-245. https://dx.doi.org/10.3354/ame033239
In: Aquatic Microbial Ecology. Inter-Research: Oldendorf/Luhe. ISSN 0948-3055; e-ISSN 1616-1564, more
Peer reviewed article  

Available in  Authors | Dataset 

Keywords
    Aplysina Nardo, 1834 [WoRMS]; Pirellula Schlesner & Hirsch, 1987 [WoRMS]; Porifera [WoRMS]
    Marine/Coastal
Author keywords
    Pirellula · Planctomycete · Aplysina · Sponge · Porifera · Sponge-associated microorganism · 16S rDNA

Authors  Top | Dataset 
  • Pimentel-Elardo, S.
  • Wehrl, M.
  • Friedrich, A.B.
  • Jensen, P.R.
  • Hentschel, U.

Abstract
    There is mounting molecular evidence that bacteria belonging to the phylum Planctomycetes are abundant in marine sponges including members of the genus Aplysina. In an attempt to culture planctomycete bacteria from Aplysina sponges, 116 bacterial strains were isolated on selective oligotrophic media. Screening of the strain collection by fluorescence in situ hybridization with the planctomycete-specific probe Pla46 yielded 3 positive candidates. Nearly complete sequencing of the respective 16S rRNA genes revealed that the isolates were affiliated with 2 distinct clusters of the genus Pirellula: 1 isolate was obtained from a Mediterranean sponge, 1 from a Caribbean sponge and a third from Caribbean seawater. To our knowledge this is the first report of cultured Planctomycetes from marine sponges. The isolates grew slowly on oligotrophic media and failed to grow on nutrient-rich media. Pirellula sp. Strain 797 was pink-pigmented while the other 2 isolates, 16 and 81, were non-pigmented. Transmission electron microscopy revealed a pear- or droplet-shaped cell morphology that is characteristic of the genus Pirellula. The application of strain-specific oligonucleotide probes to sponge tissue cryosections showed that the isolates contribute only a minor fraction to the total microbial community that is associated with Aplysina spp. sponges.

Dataset
  • CorMedNet- Distribution and demographic data of habitat-forming invertebrate species from Mediterranean coralligenous assemblages between 1882 and 2019., more

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors | Dataset