The dataset comprises data on the substrate type, temperature, salinity and benthos from 21 samples colected in NW Black Sea in the period 9-24 May 1997. Benthos samples have been take with a cylindrical plagtic bottle with cut off bottom named "Bucov" (S=1/172 cm2, h=10-12 cm). The sediment was preserved in buffered formalin 4% , then sieved through a 1 and 0.25 mm mesh sieve and subsequently stored in 70% ethanol. The database comprises taxonomic and quantitative data (density and wet weight biomass per square meter) for 75 macrobenthic species and 2 supraspecific groups.