Technical details 

Data preprocessing

Raw FlowCam output data is fully processed using in-house datapipelines, the VisualSpreadsheet software is only used for data acquisition during the lab run of the sample. Raw images and binary images are never saved during the FlowCam run, we only work on the image collages saved at the end of the run. Single images are cut from these collages using each image coordinates width and height pulled from the .lst file using in-house python code. The background of the images is not removed. These images are then predicted and annotated in-house at VLIZ.

Data splitting

The training dataset is 80% used for training, 10% for validation and 10% for prediction. 

Classes, labels and annotations

The dataset comprises 337,514 images distributed across 95 classes, with each class containing a minimum of 100 and a maximum of 10,000 images. Taxonomic coverage of the dataset comprises mainly of diatoms, dinoflagellates and cilliates, but to a lesser extent also zooplankton and other protists.

Parameters

The images are read using cv2.imread and the values are used as parameters.

Metadata Parameter Descriptions:

•    image_path: The relative path to the image file showing the plankton or particle, usually structured by taxon or project folder.
•    sample_datetime: The exact date and time (in YYYY-MM-DD HH:MM:SS.sss format) when the sample was acquired using the imaging instrument.
•    flowcam_version: The software or hardware version of the FlowCAM instrument used to capture the image and process the sample.
•    station: The sampling location code where the image was taken. These station codes typically refer to predefined geographic or monitoring points in the field.
•    accepted_label: The final validated taxonomic label (e.g., genus or species) assigned to the organism or particle in the image, often based on expert review.
•    accepted_aphia_id: The unique identifier (AphiaID) corresponding to the accepted taxonomic label in the World Register of Marine Species (WoRMS) database, which ensures standardized taxonomic reference.
•    original_reference_id: A unique identifier (often a UUID) assigned to the image or sample in the original classification system (e.g., EcoTaxa), useful for traceability and linking back to the source record.

Data sources

Images are collected during the monthly monitoring of phytoplankton communities in the Belgian Part of the North Sea during the LifeWatch multidisciplinary campaigns by FlowCam VS-4 benchmodel (Fluid Imaging Technologies, Yarmouth, Maine, U.S.A.).

Data quality

All images are predicted and subsequently manually validated to ensure the quality of the trainingset.

Image resolution

The size range imaged is 55-300µm. Images are acquired using a Sony XCD SC90 digital gray-scale camera. Images are during training of CNN resized to 100px by 100px.

Spatial coverage 

The data comes from a number of fixed stations in the Belgian Part of the North Sea (BPNS). 

Nine stations onshore are visited monthly:

Eight additional offshore stations are visited seasonally: