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Supplementary information accompanying the paper "Nematodes facilitate bacterial-mediated biogeochemical processes in marine sediments: a meta-transcriptomics approach"
Citable as data publication
Guden, R.M.E.; Haegeman, A.; Ruttink, T.; Moens, T.; Derycke, S.; Marine Biology Research Group - Ugent; Institute for Agricultural and Fisheries Research (ILVO): Belgium; (2022): Supplementary information accompanying the paper "Nematodes facilitate bacterial-mediated biogeochemical processes in marine sediments: a meta-transcriptomics approach". Marine Data Archive. https://doi.org/10.14284/575

Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Description
This study used metatranscriptomics to investigate taxonomic and functional shifts in marine sediment bacterial communities in response to the addition of a nematode community. The scripts and data in this repository are accompanying the paper "Nematodes facilitate bacterial-mediated biogeochemical processes in marine sediments: a meta-transcriptomics approach" by Rodgee Mae Guden, Annelies Haegeman, Tom Ruttink, Tom Moens and Sofie Derycke (Ghent University / ILVO, Belgium). The scripts are also available on Gitlab, with an overview of the bioinformatics pipeline. more

Our study investigated the taxonomic and functional shifts in benthic bacterial communities in response to the addition of a nematode community using a metatranscriptomic approach. We created 10 microcosms comprising sediment and natural bacterial populations, and extracted RNA at the start of the experiment. Natural nematode communities from an intertidal mudflat were then added to five of the microcosms. After seven days of incubation, RNA was extracted from the microcosms to perform metatranscriptomics analysis to investigate the taxonomic and functional composition of the samples.

Scope
Themes:
Biology > Benthos > Meiobenthos, Biology > Ecology - biodiversity
Keywords:
Marine/Coastal, Bacteria, Carbon and nitrogen cycling, Cluster of Orthologous genes (COGs), Ecosystem functioning, Functional annotation, Marine biogeochemistry, Metatranscriptomics, Nematode composition, Sediment microbiome, Taxonomic composition of bacterial community, ANE, Netherlands, Westerschelde, PaulinaPolder

Geographical coverage
ANE, Netherlands, Westerschelde, PaulinaPolder

Contributors
Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Onderzoeksgroep Mariene Biologie (MARBIOL), moredata creator
Vlaamse overheid; Beleidsdomein Landbouw en Visserij; Instituut voor landbouw-, visserij en voedingsonderzoek (ILVO), moredata creator

Dataset status: Completed
Data type: Data
Data origin: Research
Metadatarecord created: 2022-09-13
Information last updated: 2022-09-15
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy