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Improvement to the HPLC-fluorescence analysis method for the determination of acid DSP toxins
Pereira, A.; Klein, D.; Sohet, K.; Houvenaghel, G.; Braekman, J.C. (1995). Improvement to the HPLC-fluorescence analysis method for the determination of acid DSP toxins, in: Lassus, P. et al. (Ed.) Harmful marine algal blooms: Proceedings of the Sixth International Conference on toxic marine phytoplankton = Proliférations d'algues marines nuisibles: Sixiéme Conférence Internationale sur le phytoplancton toxique, October 18-22, 1993, Nantes, France. pp. 333-338
In: Lassus, P. et al. (Ed.) (1995). Harmful marine algal blooms: Proceedings of the Sixth International Conference on toxic marine phytoplankton = Proliférations d'algues marines nuisibles: Sixiéme Conférence Internationale sur le phytoplancton toxique, October 18-22, 1993, Nantes, France. Technique et Documentation - Lavoisier/Intercept: Paris. ISBN 2-85206-972-5. XXIII, 878 pp., more

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Document type: Conference paper

Keywords
    Algal blooms
    Analysis > Biochemical analysis
    Analytical techniques > Chromatographic techniques
    Analytical techniques > Spectroscopic techniques > Fluorescence spectroscopy
    Materials > Hazardous materials > Biological poisons
    Marine/Coastal

Authors  Top 
  • Pereira, A.
  • Klein, D.
  • Sohet, K.
  • Houvenaghel, G., more
  • Braekman, J.C., more

Abstract
    HPLC/FD analysis of okadaic acid (OA) and dinophysistoxin 1 (DTX1) after derivatization with 9-anthryldiazomethane (ADAM) is the most practical and promising approach to detect these toxins in contaminated seafoods. Several parameters of the procedure have been systematically optimized. This has led us to propose an improved procedure, which when applied to mussel hepatopancreas extracts spiked with known amounts of toxins, gives reliable and reproducible results.

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